| 2024 Emilia Romagna MotoGP, Misano – Practice Results |
| Pos | Rider | Nat | Team | Time/Diff | Lap | Max |
| 1 | Francesco Bagnaia | ITA | Ducati Lenovo (GP24) | 1’30.286s | 18/23 | 299k |
| 2 | Jorge Martin | SPA | Pramac Ducati (GP24) | +0.198s | 22/22 | 299k |
| 3 | Marc Marquez | SPA | Gresini Ducati (GP23) | +0.299s | 20/25 | 298k |
| 4 | Enea Bastianini | ITA | Ducati Lenovo (GP24) | +0.321s | 23/27 | 300k |
| 5 | Fabio Quartararo | FRA | Monster Yamaha (YZR-M1) | +0.600s | 23/26 | 294k |
| 6 | Franco Morbidelli | ITA | Pramac Ducati (GP24) | +0.646s | 24/26 | 298k |
| 7 | Marco Bezzecchi | ITA | VR46 Ducati (GP23) | +0.678s | 26/26 | 299k |
| 8 | Maverick Viñales | SPA | Aprilia Racing (RS-GP24) | +0.704s | 25/26 | 298k |
| 9 | Pedro Acosta | SPA | Red Bull GASGAS Tech3 (RC16)* | +0.705s | 20/21 | 299k |
| 10 | Aleix Espargaro | SPA | Aprilia Racing (RS-GP24) | +0.881s | 23/24 | 296k |
| 11 | Jack Miller | AUS | Red Bull KTM (RC16) | +0.931s | 20/23 | 298k |
| 12 | Johann Zarco | FRA | LCR Honda (RC213V) | +0.964s | 24/25 | 298k |
| 13 | Fabio Di Giannantonio | ITA | VR46 Ducati (GP23) | +1.107s | 16/17 | 293k |
| 14 | Alex Marquez | SPA | Gresini Ducati (GP23) | +1.227s | 25/25 | 297k |
| 15 | Brad Binder | RSA | Red Bull KTM (RC16) | +1.362s | 5/22 | 298k |
| 16 | Miguel Oliveira | POR | Trackhouse Aprilia (RS-GP24) | +1.382s | 5/17 | 298k |
| 17 | Raul Fernandez | SPA | Trackhouse Aprilia (RS-GP24) | +1.434s | 24/25 | 296k |
| 18 | Luca Marini | ITA | Repsol Honda (RC213V) | +1.479s | 26/26 | 297k |
| 19 | Joan Mir | SPA | Repsol Honda (RC213V) | +1.638s | 24/25 | 301k |
| 20 | Augusto Fernandez | SPA | Red Bull GASGAS Tech3 (RC16) | +1.814s | 19/20 | 298k |
| 21 | Takaaki Nakagami | JPN | LCR Honda (RC213V) | +1.924s | 4/24 | 296k |
